Journal: Molecular medicine reports

Article Title: CXC195 induces apoptosis and endoplastic reticulum stress in human hepatocellular carcinoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.

doi: 10.3892/mmr.2015.4479

Figure Lengend Snippet: Figure 2. Effects of CXC195 on the proliferation of HepG2 cells. (A) Cell cycle distribution was examined using flow cytometry. (B and C) Protein expres sion levels of PCNA, Ki67, CDK4, cyclin D1, p21, p27 and p53 were determined using western blot analysis. GAPDH was used to confirm equal protein loading. Data are presented as the mean ± standard deviation (n=6). *P<0.05 and **P<0.01, vs. control. PCNA, proliferating cell nuclear antigen; CDK4, cyclin‑dependent kinase 4; Con, control.

Article Snippet: The membrane was blocked with 5% skimmed milk and incubated with PBS containing Tween 20 and the following primary antibodies at 1:500 dilution: Monoclonal/polyclonal mouse/rabbit anti-human proliferating cell nuclear antigen (PCNA) (cat no. sc-25280), p21 (sc-397), p27 (sc-393380), p53 (sc-126), Bcl-2 (sc-7382), Bcl-2-associated X (Bax) (sc-7480), AIF (sc-13116), Cyt-c (sc-13561), cyclooxygenase (COX)4 (sc-292052), tubulin (sc-9104), histone (sc-10806), glucose‐regulated protein (GRP)94 (sc-11402), GRP78 (sc-1050), CCAAT-enhancer-binding protein homologous protein (CHOP) (sc-575) and GAPDH (sc-365062) (Santa Cruz Biotechnology, Dallas, TX, USA) and cyclin-dependend kinase (CDK)4 (cat no. 12790), cyclin D1 (1044), phosphorylated (p)-PERK (3179), PERK (3192), eIF2α (9722), p-eIF2α (3597), ATF4 (11815), IRE1α (3294), p-ASK (3765), ASK (37626S), p-p38 (4511), p38 (9213), ATF6 (11815S), p-PI3K (4288S), PI3K (4249S), p-AKT (4060P), AKT (#2920S), mammalian target of rapamycin (mTOR) (2983S) and p-mTOR (5536S) (Cell Signaling Technology, Inc., Danvers, MA, USA) overnight at 4 ̊C.

Techniques: Flow Cytometry, Western Blot, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Action Mechanism of Fibroblast Growth Factor-2 (FGF-2) in the Promotion of Periodontal Regeneration in Beagle Dogs

doi: 10.1371/journal.pone.0131870

Figure Lengend Snippet: (A): Schematic of the observation regions; outside and margin zones (a), center zone (b), and upper zone (c) of the defect. (B–I): Representative histological overviews from control (B, D, F, H) and FGF-2 (C, E, G, I) groups are shown at 3 days (B, C) and 7 days (D–I). Immunostaining of PCNA and higher magnifications of the rectangular areas in B–I are shown in B’–I’. cl, clot; gt, granulation tissue; ct, connective tissue; b, existing bone; g, gingival connective tissue; black arrowhead, positive cells expanded from the existing bone marrow. Scale bar represents 100 μm.

Article Snippet: Sections were immunostained with a rabbit polyclonal anti-human PCNA antibody (Santa Cruz Biotechnology Inc., Dallas, Texas, USA) using a Histofine SAB-PO (R) kit (Nichrei Bioscience, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

Techniques: Control, Immunostaining

Journal: PLoS ONE

Article Title: Action Mechanism of Fibroblast Growth Factor-2 (FGF-2) in the Promotion of Periodontal Regeneration in Beagle Dogs

doi: 10.1371/journal.pone.0131870

Figure Lengend Snippet: (A): Schematic of the measurement regions. The defect was compartmentalized into four zones: outside, margin, center, and upper. We evaluated 4 mm 2 in the outside zone, 6 mm 2 in the margin zone, 1 mm 2 in the center zone, and 3 mm 2 in the upper zone per defect. (B–E): Y-axis values represent the number of PCNA-positive cells per 1 mm 2 . All results are presented as the mean ± SD (n = 4) (*, p <0.05; **, p <0.01 compared with the control, t-test).

Article Snippet: Sections were immunostained with a rabbit polyclonal anti-human PCNA antibody (Santa Cruz Biotechnology Inc., Dallas, Texas, USA) using a Histofine SAB-PO (R) kit (Nichrei Bioscience, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

Techniques: Control

Journal: PLoS ONE

Article Title: Action Mechanism of Fibroblast Growth Factor-2 (FGF-2) in the Promotion of Periodontal Regeneration in Beagle Dogs

doi: 10.1371/journal.pone.0131870

Figure Lengend Snippet: (A): Schematic of the observation regions; defect bottom around the existing PDL (a) and root surface (b). (B–G): Representative histological overviews from control (B, D, F) and FGF-2 (C, E, G) groups are shown at 3 days (B, C) and 7 days (D–G). Immunostaining of PCNA and higher magnifications of the rectangular areas in B–G are shown in B’–G’. cl, clot; gt, granulation tissue; ct, connective tissue; b, existing bone; d, dentin; p, existing PDL; c, cementum; arrowheads, positive cells extending from the existing PDL. Scale bar represents 100 μm. (H): Total number of proliferating cells in a 0.25 mm width from the root surface. The number of PCNA-positive cells was counted from the defect bottom to the apical on the root surface. The results are presented as the mean ± SD (n = 4) (***, p <0.001 compared with the control, t-test).

Article Snippet: Sections were immunostained with a rabbit polyclonal anti-human PCNA antibody (Santa Cruz Biotechnology Inc., Dallas, Texas, USA) using a Histofine SAB-PO (R) kit (Nichrei Bioscience, Inc., Tokyo, Japan) according to the manufacturer’s protocol.

Techniques: Control, Immunostaining